The race at the Lusail International Circuit, originally scheduled for April, was postponed to November 6-8 due to the regional conflict.
"MotoGP has provided teams and riders with every assurance available from the State of Qatar, the circuit... and our own ongoing risk assessment processes," a MotoGP spokesperson told Reuters.
"We fully respect the concerns raised and highlight that safety remains the priority for everyone involved."
However, the spokesperson said the expectation of a zero-risk environment was not realistic anywhere in the world by any government or organiser.
"Over the past months, we have maintained continuous dialogue with teams and riders, sharing the latest information available, providing direct access to documents and answering questions openly and transparently."
Carlos Ezpeleta, deputy CEO of MotoGP, visited Qatar on Thursday where the circuit's president Abdulrahman Al-Mannai said they were ready to welcome the riders, teams and fans for the 20th round of the season.
However, Spain's embassy in Qatar has issued a notice urging its citizens not to travel to the Gulf nation unless it was absolutely necessary.
Spaniards make up the largest share of riders on the grid, including world champion Marc Marquez.
Evacuation plan in place
A MotoGP source said Qatar has guaranteed teams would not be stranded in case of escalation in the conflict and they could leave the country safely in time for the final two rounds in Portugal and Spain later in November.
"Additional impossible asks at the last moment go beyond what can realistically be guaranteed by any event organiser, government or sporting body," the spokesperson added.
"Our position is based solely on the most up-to-date intelligence, expert advice and assurances available to us."
The source said championship leader Marquez, a seven-times MotoGP champion, was one of the riders seeking more assurances.
Marquez has not publicly stated his position on travelling to Qatar but Ducati team manager Davide Tardozzi defended the Spaniard after he came under criticism for not taking a stance.
"I have read that all the blame has been placed on Marc, as if he were the one who did not want to go to Qatar. That is not the case," he told Sky Sport.
"Marc simply asked, as many other riders did, for an organisational safety plan - nothing more, nothing less. He did not say, 'I don't want to go'.
"There are circumstances that could make you think it would be better not to go. But if the necessary safety conditions are in place, including an evacuation plan and guarantees that there will be no problems if something happens, then we will go."
Reuters has contacted Ducati and Marquez for comment.
Aprilia keen to race in Qatar
Aprilia are keen to race in Qatar with Spanish rider Jorge Martin, second in the standings behind Marquez after withdrawing from the Malaysian Grand Prix due to a wrist injury.
"We need to turn the page and look to the next chapter, Qatar, hoping it goes better, and keep this fight going to the last," Aprilia's technical director Fabiano Sterlacchini said.
"This setback has certainly made things harder for us."
The MotoGP source confirmed that no decision has been taken about sporting sanctions if teams or riders do not participate in Qatar.