The first round saw several shock results, with Excelsior '31 from Rijssen taking down Eredivisie side ADO Den Haag on Tuesday, SC Genemuiden knocking out FC Dordrecht in a thriller on Wednesday, and Keuken Kampioen Divisie leaders Heracles Almelo shown the door by De Treffers on Thursday.
Missed the action? Catch up on all the action with our daily roundups:
In the second round, amateur teams may not be as lucky, as their home advantage no longer applies beyond the first round. In the first round, amateur teams always played at home when drawing a professional team, but that advantage is no longer on their side.
The second round also sees the entrance of the six Dutch clubs that are active in Europe: PSV, Feyenoord, NEC Nijmegen, AZ, Ajax, and FC Twente all enter the KNVB Beker in the second round.
The second-round draw will feature 32 total teams, including 10 amateurs and just 8 teams from the second division:
Eredivisie: PSV, Feyenoord, AZ, NEC, Ajax, FC Twente, Willem II, SC Cambuur, SC Heerenveen, FC Utrecht, Go Ahead Eagles, Fortuna Sittard, Excelsior
Keuken Kampioen Divisie: RKC Waalwijk, FC Eindhoven, FC Den Bosch, Roda JC, Almere City, NAC Breda, , TOP Oss, FC Emmen
Tweede Divisie: Quick Boys, AFC, De Treffers, GVVV, HSV Hoek, RKAV Volendam
Derde Divisie: Purmersteijn, Genemuiden, Excelsior '31, VVSB
All second-round matches will be played on December 1st, 2nd and 3rd.